Mass Spectrometry Facility

Welcome to the Biological Mass Spectrometry Facility (BMSF) of Robert Wood Johnson Medical School and Rutgers, The State University of New Jersey. The facility is directed by Dr. Haiyan Zheng and is located in Room 010 of Center for Integrative Proteomics Research (CIPR) building on the RWJMS/Busch Campus in Piscataway.

For users who prepare publications using data generated using the following instruments from our facility, please kindly acknowledge our facility (the Biological Mass Spectrometry Facility (BMSF), of Robert Wood Johnson Medical School and Rutgers The State University of New Jersey, Piscataway, NJ 08854) and appropriate instruments:

Tims TOF HT: 1S10OD036226-01

Eclipse: 1S10OD025140-01

QE HF: 1S10OD016400-01

174 Frelinghuysen Road Piscataway, NJ 08854
848-445-5297
haiyanz@cabm.rutgers.edu

Instruments

Bruker TimsTOF HT with Thermo Vanquish NEO nano LC

This set of high-end instruments is funded by the NIH instrument grant (1S10OD036226-01). Since its installation in March 2025, it has been the work horse instrument of our facility. Using parallel accumulation-serial fragmentation (PASEF) technology, the instrument is capable of 4-D proteomics (ion mobility, retention time, mass-to-charge ratio and intensity). It enables more precise identification and quantitation of proteins within complex samples such as cell lysate, allows more sensitive detection of protein binding partners and their stoichiometries as well as precise site locations for post-translational modification studies. This instrument typically is operated by facility staff on behalf of users.

Thermo Orbitrap Eclipse Tribrid Mass Spectrometer with a Dionex U-3000 Rapid Separation nano LC system

This instrument was installed in October 2019 and is interfaced with a tandem Dionex nano RSLC system. The Eclipse combines quadrupole, ion trap and Orbitrap mass analyzers and is designed for a broad range of high-resolution, qualitative and quantitative applications. An application specific to the Eclipse and other Tribrid mass spectrometers is the ability to measure reporter ion intensities at the MS3 level in isobaric labeling proteomics studies. Compared to traditional MS2-level quantitation, MS3-level measurement results in significantly increased accuracy of protein quantitation, which is particularly helpful in large-scale proteome-wide quantitative studies. The instrument typically is operated in the nanoLC-mode by facility staff.

Thermo Q Exactive HF mass spectrometer with a Dionex U-3000 Rapid Separation nano LC system

This instrument was installed in December 2014 and is interfaced with a tandem Dionex nano RSLC system. The mass spectrometer uses a quadruple for precursor ion selection with HCD fragmentation and high-resolution, Orbitrap detection. Multiplex scanning (MSX) further increases duty cycle for targeted MS/MS. This instrument has a broad range of qualitative and quantitative applications and is operated by Facility staff.