James (Jimmy) Siclari, PhD

Jimmy’s PhD work focused on the one-component signaling class of bacterial proteins in Dr. Kevin H. Gardner’s lab at the CUNY Advanced Science Research Center. Using a suite of structural biology and biochemistry techniques including NMR, X-ray crystallography, and hydrogen-deuterium-exchange (HDX) mass-spectrometry, Jimmy pursued the signaling mechanisms of bacterial sensor proteins with varying stimuli (redox state, ligand binding, temperature, and blue-light) that share many of the same core signal propagation mechanisms. 

In joining Dr. Ann Stock’s lab at the CABM Jimmy hopes to elucidate the structure-function relationship and ligand dependent gene regulation of polysaccharide sensing hybrid two-component systems (HTCS) in Bacteroides, a major component of the human gut microbiome.